Figure 7.

Linear correlation between the percentage of cells with supernumerary centrosomes and the incidence of mitotic catastrophe. Wild-type (WT) and p53^−/− diploid human colon carcinoma HCT 116 cells that constitutively co-express biosensors for the detection of chromatin status and centrosomes were cultured for 8 h in the absence (Control) or in the presence of paclitaxel (PTX), dimethylenastron (DIMEN), nocodazole (NDZ) or 7-hydroxystaurosporine (UCN-01), and then monitored by videomicroscopy for the automated quantification of the number of centrosomes per cell and of mitotic catastrophe. In both WT (a) and p53^−/− (b) diploid cells, the percentage of cells characterized by supernumerary centrosomes linearly correlated with the incidence of mitotic catastrophe. Data are mean values from three independent experiments. m=slope; R²=coefficient of determination