Figure 6.

Evaluation of the myeloid differentiation markers' expression by quantitative real-time PCR. The figure shows the decrease of the erythrocyte plasma membrane glycoprotein Rh-associated glycoprotein, Rhag; hemoglobin alpha 1, Hba1 and of the Beta-globin gene expression regulator, Kruppel-like factor 1(Klf1). Alongside, it shows the increase in the granulocytic markers myeloperoxidase (Mpo) and elastase 2 (Ela2) together with the megakaryocytic-determining factor friend leukemia virus integration 1 (Fli1). Nuclear factor (erythroid-derived 2), Nfe2, which is involved in the late differentiation phase of platelet assembly and release, as expected, was not significantly upregulated and neither were the monocytic markers interferon regulatory factor 8 (Irf8), Kruppel-like factor 4 (Klf4) and v-Maf musculoaponeurotic fibrosarcoma oncogene homolog B (Mafb). Delta-delta-CTs and RQs were calculated for each detector using as calibrator the nucleofection solution-treated sample (nuc) and glyceraldehyde-3-phosphate dehydrogenase as endogenous control. Mean±S.D. from three independent experiments