Figure 7.

Effects of inducible PRELI/LEA⁻ expression and transient PRELI knockdown. (a) Flow cytometric analyses to monitor mitochondrial ΔΨ_m (upper histograms) and ROS production (center and lower histograms) in Tet-ON JY cells without (non-induced) or with induced PRELI/LEA⁻ expression (DOX-induced), in the absence (red-dotted lines in all the panels) or presence of either CCCP (blue-dotted lines in the panels on the left) or rotenone (blue-dotted lines in the panels on the right). Alterations in ΔΨ_m are reported as the log of CMxROS (FL2) fluorescence shift and ROS changes are monitored by the change in the log value of FL3/HEt and FL2/CM-H₂-DCFDA fluorescence, which respectively measure O₂ and H₂O₂ levels. The numbers represent the percentage of cellular events. (b) Cell-cycle histograms revealing the differential accumulation of propidium iodide-stained DNA fragments at the sub-G stage between JY cells transiently transfected with either non-targeting siRNA control or experimentally selected PRELI-specific siRNA 327 (denotes the relative position of the targeted PRELI mRNA sequence) after incubation in the absence (untreated) or presence of STS (STS-treated). The numbers indicate the percentage of cells undergoing DNA fragmentation