Figure 5.
JNK phosphorylation of YAP enhances the stabilization of ΔNp63α by direct binding. (a) HaCaT cells were transfected with control siRNA (siCTL) or YAP siRNA (siYAP) and 48 h later irradiated with 50 J/m2 UV. Cells were harvested at the indicated time points after UV irradiation and analyzed by Western blotting using the indicated antibodies. (b) HaCaT cells were irradiated with 50 J/m2 UV and harvested for IP 30 min, 1, 2 and 6 h later. Endogenous YAP was immunoprecipitated and IP inputs and eluates were analyzed by Western blotting using the indicated antibodies (c) HaCaT cells were transfected with control siRNA (siCTL) or ITCH siRNA (siITCH) and 72 h later the cells were harvested for IP of endogenous p63. p63 IP eluates and input lysates were analyzed by Western blotting using the indicated antibodies. (d) H357 cells were transfected with control siRNA (siCTL) or YAP siRNA (siYAP) and after 72 h irradiated with 50 J/m2 UV. H357 cells were then harvested 4 h after irradiation and analyzed by Western blotting using the indicated antibodies. (e) H357 cells were transfected with control siRNA (siCTL) or YAP siRNA (siYAP) and 72 h later the cells were harvested for IP of endogenous p63. p63 IP eluates and input lysates were analyzed by Western blotting using the indicated antibodies. (f) U2OS cells were co-transfected with pcDNA-ΔNp63α and either Flag-EV, Flag-YAP, Flag-mut2AYAP or Flag-mut2DYAP. Flag IP was performed and IP inputs and eluates analyzed by Western blotting using the indicated antibodies