Figure 6.
Expression of ΔNp63α uncovers the role of YAP in UV-induced apoptosis. (a) Duplicate lysates from BWT and HaCaT cells were immunoblotted for p63 with either p63α or pan-p63 antibodies as indicated and also β-tubulin expression for loading control. (b) HaCaT cells were transfected with control siRNA (siCTL) or an siRNA to p63 (sip63) and after 48 h were irradiated with 50 J/m2 UV. Cells were harvested at the indicated time points and analyzed by Western blotting using the antibodies shown. (c) BWT cells were transfected with pcDNAHA empty vector control (EV) or pCDNAHA-ΔNp63α (p63) and after 48 h UV-irradiated at 30 J/m2. The cells were harvested after 6 h and the expression of the indicated proteins analyzed by Western blotting