Figure 1.

Downregulation of MCL-1 enhances dexamethasone-induced lethality. Left panel: CEM cells were infected with lentiviruses expressing shRNAs for non-targeting control, BIM, MCL-1, or BCL-2. Puromycin-resistant cells were pooled after each infection. Equal amounts of total cell extracts were subjected to western blotting with the indicated antibodies. Right panel: Cells were treated with 100 nM Dex for 48 h. The percentage of apoptotic cells was determined by Annexin V-propidium iodide (PI) staining followed by FACS analysis. Values represent the mean±S.D. of three independent experiments