Figure 6.

GX15-070-induced apoptosis is BAK-dependent. (a) CEM cells were infected with lentiviruses expressing shRNAs for non-targeting control, BAX, or BAK. Puromycin-resistant cells were pooled after each infection. Downregulation of BAX or BAK expression was monitored by western blotting (in sets). Cells were treated with 100 nM GX15-070 for 48 h and the percentage of cell death was determined by Annexin V-PI staining followed by FACS analysis. Values represent the mean±S.D. of three independent experiments. (b) Cells harboring control, BAX, or BAK shRNA were treated with 100 nM GX15-070 for 32 h. Caspase-3 cleavage and LC3 conversion was determined by western blot analysis