Table 1. Kinetics of the parasites' death under H₂O₂ treatment.

	AnV (+) 7-AAD (−)	AnV (+) 7-AAD (+)	AnV (−) 7-AAD (+)
Wild-type (−) H2O2	0.033	0.007	0.004
Wild-type (+) H2O2	0.14	0.42	0.79
CPC KO (−) H2O2	0.29	0.07	0.02
CPC KO (+) H2O2	0.29	0.28	0.18
CPC KO/+ (−) H2O2	0.16	0.04	0.02
CPC KO/+ (+) H2O2	0.32	0.45	0.53

Wild-type parasites (WT), cathepsin B-deficient parasites (cpc KO) and cpc KO complemented strain (cpc KO/+) that contain an episomal copy of cpc were not treated (−) or treated (+) with 1 mM H₂O₂ for 1 h. Cells were stained with fluorescein isothiocyanate (FITC)-labeled Annexin V and 7-AAD to distinguish between live (AnV−/7AAD−), apoptotic (AnV+/7AAD−, early apoptotic cells; AnV+/7AAD+, late apoptotic/necrotic) and necrotic cells (AnV−/7AAD+) as depicted in Figure 6. Values correspond to ratios of percentage of AnV+/7-AAD−, AnV+/7-AAD+ and AnV−/7-AAD+ cells to percentage of AnV−/7-AAD−