Figure 12.

Optically determined binding rates of multiple lipid probes in BHK cells before and after inducing MEND with 150 µM TX100. Binding rates of dyes are assumed to be proportional to the rate of fluorescence increase over 20 s upon applying dyes. The gray dashed lines alongside of the fluorescence records indicate the average rate of fluorescence increase. (A) An experiment using 1 µM C5-Bodipy-Gm1. (B) An experiment using 5 µM C16:0-NBD-PE in the presence of 10% DMSO to promote lipid solubility. (C) Summary of results showing capacitance loss in white and binding rates of fluorescent probes in gray. Only the styryl FM dye and the C16:0-NBD-PE are significantly affected by TX100-induced MEND.