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. 2011 Feb;137(2):155–172. doi: 10.1085/jgp.201010470

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© 2011 Hilgemann and Fine

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Figure 6. — Analysis of DPA-capacitive signals before and after MEND induced by a Ca transient in the presence of 1 mM spermidine and 6 mM ATP. MEND amounts to 68% of C_m. DSF, 4.0. (A) 2 µM DPA is applied twice before MEND and twice after MEND for times of 6–10 s. During each application of DPA, voltage is stepped to −150 mV and then ramped to +80 mV to determine the capacitance–voltage relation of DPA. (B and C) Capacitance records indicated by “before” and “after” in A. The magnitudes of DPA-capacitive signal and the time constants of the rising and falling signals (0.67–0.82 s) are not detectably changed. (D) The magnitudes of DPA capacitance–voltage relations are not detectably changed; the peak capacitance undergoes a small 8-mV shift to more negative potentials.