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. Author manuscript; available in PMC: 2011 Feb 2.
Published in final edited form as: Aging Cell. 2008 Sep 8;7(6):805–812. doi: 10.1111/j.1474-9726.2008.00438.x

Figure 6.

Figure 6

Measurement of endothelial cell (EC) nuclear protein expression. A: Representative EC immunostained for the p65 subunit of the nuclear transcription factor NFκB and visualized by fluorescent microscopy. B: Nucleus of the same cell is visualized by DAPI nuclear stain. C: Overlay of nuclear stain with that of total EC NFκB p65. The border of the nucleus is outlined using this overlay image. D: Fluorescent intensity of the cell within the border of the nucleus is measured to determine nuclear NFκB p65 protein expression.