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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Horm Mol Biol Clin Investig. 2010 Aug 1;2(2):235–243. doi: 10.1515/HMBCI.2010.025

Figure 2.

Figure 2

E2-mediated upregulation of XBP1 in ECC1 cells. ECC1 cells treated with E2 (1 nM) for 2, 4, 8, 16, 24 or 48 h and expression of XBP1 was measured using quantitative real-time PCR and compared with vehicle-treated cells (A). ECC1 cells were treated with CHX (10 μg/mL), DRB (75 mM) or FUL (1 mM) in absence or presence of E2 (1 nM) for 24 h and expression of XBP1 was assessed using real-time PCR (B). SKBR-3 and MDA-MB-231 cells were treated with E2 (1 nM) or vehicle (0.1% ethanol) for 24 h and expression of XBP1 was measured using quantitative real-time PCR and compared with vehicle-treated cells (C). Total RNA from MCF7 and MCF:5C was isolated and expression of XBP1 was measured using quantitative real-time PCR relative to MCF7 cells (D). MCF7:5C cells were treated with vehicle (0.1% ethanol) or E2 (1 nM) for 48 h and expression of XBP1 was measured using quantitative real-time PCR and compared with vehicle-treated cells (E). *p<0.05 compared with vehicle-treated group (B). **p<0.05 compared with MCF7 cells (D) or vehicle-treated group (E).