TABLE 2. Design of Study of Henipavirus F.
Immunization of Mice with Wild-Type VRP Expressing HeVF or NiVF.
| Replicon | Transgene | Dose (IU) a | Group Designation |
|---|---|---|---|
| Wild Type | HeVF | 50 μl VRPs b | V-HeVF |
| Wild Type | NiVF | 50 μl VRPs b | V-NiVF |
| Wild type | R2gp160 | 1 × 106 | V-R2gp160 |
| None | N/A | N/A | Control |
Infectious units.
VRP dose for each individual mouse per group was the same, ensured by using a fixed homogenous pool of VRP preparation for all immunizations; replicon particle titers could not be calculated due to lack of a suitable antibody for use in immunofluorescent assays.