Proposed inhibition by darkness of steps leading to isoprene production in the short term. Steps in the MEP pathway expected to be most susceptible to depletion of reducing power upon darkening are indicated here. 1,3-BPG, 1,3-Bisphosphoglycerate; DXR, deoxyxylulose phosphate reductoisomerase; DXS, deoxyxylulose phosphate synthase; ETC, electron transport chain; HDS, hydroxymethylbutenyl diphosphate synthase; MEcDP, methylerythritol 2,4-cyclodiphosphate; PEP, phosphoenolpyruvate. ATPs are omitted here for simplicity. Steps between 3-PGA and GAP are reversible. Coupling of NADPH is only shown in the forward reaction for simplicity.