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Isolation of HPR3 knockout mutants. A, Structure of the HPR3 gene and positions of the T-DNA insertions of two independent lines (gray bars represent the exons). B, HPR3-specific transcripts were undetectable in both homozygous lines but were found to be present in wild-type plants. Whole-leaf RNA was isolated and analyzed by RT-PCR using S16 mRNA as a control for the integrity of generated cDNA. This result was obtained for six plants (each line) and three technical replicates.
