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Crossing of hpr1-1, hpr2-1, and hpr3-1 leads to functional inactivation of the three HPR genes. RT-PCR analysis shows the absence of the transcripts encoded by the three HPR genes in leaves of the isolated triple mutant, whereas all three transcripts are present in the wild-type control. S16 mRNA was used as an internal control for the integrity of the produced cDNA. This result was obtained with three independent plants per line and two replicate RT-PCR analyses each.
