Figure 2.
The effect of N-terminal, C-terminal, and F-box domain deletions on the ability of HA-SLY1 and HA-SNE to rescue sly1-10. A, Shown are 30-d-old wild-type (WT) Ler, sly1-10, and sly1-10 transformed with the indicated HA fusion constructs. B, Shown are 45-d-old sly1-10 and sly1-10 HA-SNE. The HA-SNE construct partially rescued the dwarfism. C, DELLA RGA and GAI protein accumulation in wild-type Ler, ga1-3, sly1-10, and sly1-10 transformed with constructs described above was determined by anti-RGA immunoblot analysis of 40 μg of total protein extracted from 21-d-old rosette leaves.