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HA-SLY1 and HA-SNE fusion proteins interact with DELLA RGA and CUL1 protein in planta. Protein was extracted from 12-d-old sly1-10 plants transformed with the indicated constructs and incubated with HA matrix agarose in the presence of 0.1% ethanol (mock) or 100 μm GA3. Immunoprecipitated protein was loaded on an SDS-PAGE gel and detected with anti-HA antibody. Coimmunoprecipitated protein was detected with anti-RGA and anti-CUL1.
