Figure 6.

Interaction of SLY1 and GID1 in coimmunoprecipitation (Co-IP) assays. The coimmunoprecipitation experiment was performed using protein extracted from 12-d-old sly1-10 plants transformed with HA-GID1a and/or FLAG-SLY1. The protein extract was incubated with FLAG matrix agarose in the presence of 0.1% ethanol (mock) or 10 μm GA₃ and loaded on an SDS-PAGE gel. Protein-blot analysis was performed using anti-FLAG and anti-HA antibodies. The asterisk indicates a background band. Forty micrograms of total protein was loaded (input). [See online article for color version of this figure.]