Figure 1.

In vitro sprouting assay using different concentrations of GA₃. Two weeks after storage, tuber discs containing one bud each were isolated and incubated for 5 min with 5, 25, 50, or 100 μm GA₃ and water as a control. The tuber discs were kept in darkness and monitored daily for visible sprouting. A, The percentage of sprouted tuber buds after application of different GA₃ concentrations during an 8-d period. The data represent means ± sd of two independent experiments (n = 25). B, Cross sections through tuber buds before and 3 and 5 d after treatment with either 50 μm GA₃ or water. Bars = 1 mm. C, Schematic drawing of the in vitro tuber-sprouting experiment using GA₃. [See online article for color version of this figure.]