Figure 6.

In vitro tuber-sprouting assay using different concentrations of BAP. Two weeks after storage, tuber discs were isolated and incubated for 5 min with 5, 25, 50, or 100 μm BAP and water as a control. Tuber discs were kept in darkness under tissue culture conditions and scored daily for visible sprouting. A, The percentage of sprouted tuber buds after incubation with different BAP concentrations or water over an 8-d period. The data represent means ± sd of three independent experiments (n = 25). B, Cross sections through tuber buds before and 3 and 5 d after treatment with either 50 μm BAP or water. Bars = 1 mm. C, Schematic drawing of the in vitro tuber-sprouting experiment using BAP. [See online article for color version of this figure.]