Figure 2.

The three BnGPAT4 homologs encode functional GPAT enzymes but with different activities. A, The activities of the BnGPAT4 enzymes and the corresponding protein amounts detected by western blot after 18 h of Gal induction in the gat1Δ yeast expression system. B, The abundance of the BnGPAT4 polypeptides detected by western blot after 18, 20, and 22 h of Gal induction in the gat1Δ yeast expression system. C, Predicted protein topology of the three BnGPAT4 enzymes and partial amino acid sequence alignment. Conserved residues are denoted by asterisks, strongly similar amino acids by colons, weakly similar amino acids by periods, and the different amino acids among the three BnGPAT4 proteins are shown in red. [See online article for color version of this figure.]