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Cuticle permeability of B. napus gpat4 rosette leaves at different stages with the toluidine blue test. A, Comparison of the rosette leaves of wide-type and gpat4 lines after treatment with the toluidine blue solution. B, The rosette leaves at 15 d post emergence without toluidine blue treatment. The glossy area is indicated by an arrow. C, The same leaf samples in B were treated with toluidine blue. WT, Wild type. Bars = 1 cm.
