Figure 2.

Effects of candidate inhibitors on the activity of recombinant barley grain α-glucosidase. The pure enzyme was assayed using pNPG as a substrate either without inhibitors or in the presence of inhibitors at 10 μm (black bars), 1 μm (dark gray bars), 100 nm (light gray bars), or 10 nm (white bars). The specific activity of the enzyme used in this assay was 25.6 units mg⁻¹. One unit of activity is defined as the amount of enzyme that releases 2 μmol min⁻¹ Glc from maltose when using 15 mm maltose as substrate at 37°C (Naested et al., 2006). The structures and full names of inhibitors are given in Supplemental Figure S1.