Figure 2.
A stepped voltage protocol also reveals a PrsS-stimulated Ca2+-permeable conductance. A, Cartoon of the voltage step protocol. B, A family of currents obtained from an untreated pollen protoplast using the stepped voltage mode protocol (see A). C, A family of currents recorded from the same cell as in B, subjected to the voltage step protocol after exposure to incompatible PrsS. D, I-V curve for SI-induced current (obtained by subtraction of current amplitude at the end of the 4-s voltage step). Values shown are means ± se of four protoplasts.