Figure 4.

Analysis of ethylene-regulated gene expression. qRT-PCR analysis of ACO2 (A) and ETR2 (B) in various genotypes in response to ACC treatment. Plants were grown under constant blue light for 5 d on MS Suc supplemented with or without 20 μm ACC. Plants were harvested on day 5, and RNA was extracted with subsequent cDNA synthesis and analysis. All error bars represent se. All data presented are representative of at least two independent experiments, with each sample in each experiment containing three technical replicates for analysis. WT, Wild type.