Figure 2.

Apigenin induces cell-cycle arrest. (a) Number of HL60 cells in different phases of cell cycle (G₀/G₁ phase, S phase and G₂/M) as determined by propidium iodide staining followed by flow cytometry. (b) Number of TF1 cells in different phases of cell cycle. Cell-cycle analysis was performed by measuring DNA content by propidium iodide staining after treatment with specified concentrations of apigenin for 24 h. (c) Expression of p21 and c-myc (middle and lower panels) and phosphorylation of CDC2 (upper panel), key proteins in cell-cycle control in HL60 cells was determined using western blotting. (d) Expression CDK4 and 6 in TF1 cells by western blotting. Equal loading was confirmed by reprobing blots for β-actin