Figure 3.

Inhibition of c-Abl kinase activity in the context of the DNA damage response results in persistence of H2AX phosphorylation. (a) HeLa cells were pretreated with STI-571, γ-irradiated, and harvested at three time points after irradiation, and the extent of H2AX phosphorylation on Ser139 was assessed by WB. (b, c) Cells were treated as in (a) and subjected to immunofluorescence. For counting purposes, at least 50 nuclei were analyzed per sample. Data are expressed as mean±S.E.M.