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. 2010 Jan 14;1(1):e10. doi: 10.1038/cddis.2009.8

Figure 5.

Figure 5

Autophagy is required for the lifespan-extending effects of Sir-2.1 in C. elegans. (a) Longevity conferred by the overexpression of Sir-2.1 (geIn3 genotype) depends on Bec-1 expression (lifespan values: wild type, WT, control mean 14.6±0.7, max 26.0; geIn3 mean 19.5±1.1, max 33.0; bec-1(RNAi) mean 13.1±1.2, max 22.0; geIn3;bec-1(RNAi) mean 14.7±1.1, max 27.0). (b) Depletion of SIR-2.1 or Bec-1 suppresses longevity conferred by overexpression of the pyrazinamidase/nicotinamidase PNC-1 (lifespan values: WT control mean 14.6±0.7, max 26.0; Ex[pnc-1(+)] mean 18.1±1.0, max 29.0; Ex[pnc-1(+)];sir-2.1(ok434) mean 12.9±1.1, max 24.0; Ex[pnc-1(+)];bec-1(RNAi) mean 14.0±1.2, max 23.0). (c) Bec-1 deficiency suppresses longevity conferred by treatment with 100 μg/ml resveratrol (Resv) (lifespan values: WT control mean 14.6±0.7, max 26.0; WT grown on Resv mean 16.2±1.0, max 29.0; bec-1(RNAi) mean 13.1±1.2, max 22.0; bec-1(RNAi) grown on Resv mean 12.9±1.2, max 24.0). (d) Depletion of the C. elegans p53 ortholog Cep-1 does not enhance longevity conferred by Sir-2.1 overexpression (lifespan values: WT control mean 14.6±0.7, max 26.0; geIn3 mean 19.5±1.1, max 33.0; geIn3;cep-1(RNAi) mean 18.8±1.2, max 32.0). In all panels, the percentage of surviving nematodes is plotted against age (days)