Figure 3.

Apigenin-induced ‘late' Hsp27 phosphorylation is ERK-independent. Lysates from THP-1 cells were treated with diluent (lane 1), or pretreated with diluent or 25 μM PD98059 before the addition of 50 μM apigenin for 6 h (lanes 2 and 3, respectively) and immunoblotted with anti-phospho-Hsp27-pS78, anti-phospho-Hsp27-pS82, anti-phospho-Hsp27-pS15, anti-phospho-ERK, anti-total-Hsp27, anti-total-ERK antibodies. The same membrane was immunoblotted with anti-β-Tubulin antibodies as loading control. Results are a representative of four independent experiments