Figure 4.

‘Late' Hsp27 phosphorylation induced by apigenin is modulated by p38-MAPK and PKCδ. Lysates from THP-1 cells treated with 50 μM apigenin for 6 h and diluent or following 1 h pretreatment with 10 μM SB203580, 15 μM rottlerin, or both. Treatments marked with (−) denote addition of diluent. (a) Immunoblots with anti-phospho-Hsp27-pS78 and anti-total-Hsp27 antibodies. (b) Immunoblots with anti-phospho-Hsp27-pS82 and anti-total-Hsp27 antibodies. (c) Immunoblots with anti-phospho-Hsp27-pS15 and anti-total-Hsp27 antibodies. Densitometry data normalized by the loading control are represented as mean±S.E.M. (N=5, ^*P⩽0.05 and ^**P⩽0.001, NS denotes non-statistical significance)