Figure 3.

Restoration of RanGTP gradient reinstates Survivin export. (a) Immunoblotting against Mst1, Survivin, Ran and Actin was performed on lysates collected from mock, Control siRNA or Mst1 siRNA-transfected HeLa cells. (b) Rango FRET analysis was performed on Mst1 siRNA or Control siRNA-transfected HeLa cells, with or without VP16 treatment. Quantified data presented as mean±S.D. (error bar) of three independent experiments (n=120). ^**P<0.001 (Student's t-test) (c) HeLa cells transfected with Mst1 siRNA or Control siRNA before 24 h incubation with VP16. The cells were fixed and stained with anti-Survivin and mAb414. Bar, 10 μm. Images acquired with auto-optimal exposure mode