Figure 5.

Apoptotic nuclear compartmentalized Survivin has abolished cytoprotectivity. (a) Nuclearcytoplasmic fractions collected from VP16-treated cells at the indicated time points. The samples were immunoblotted against Survivin, tubulin and GCN5. (b) Procaspase-3 and cleaved Caspase-3 are predominantly cytoplasmic in VP16-treated cells indicating early apoptosis. (c) Survivin depletion from HeLa cells by 24 h Survivin RNAi transfection and immunoblotted against Survivin and Actin. (d) HeLa cells were transfected with Survivin siRNA or Control siRNA before incubation with VP16 for 24 h. Cells were fixed and stained with anti-Survivin and mAb414. Bar, 10 μm. (e) Caspase-3 activity assay. Each experiment was performed in triplicates in three independent experiments. Data presented as mean±S.D. (error bar). ^*P<0.001 (Student's t-test). (f) Hypothetical model depicting the role of nuclear export system in regulating the commitment of cells during the onset of apoptosis