Figure 5.

Induction of GRP78 and ATF6 in both liver types. (a) GRP78 mRNA expression and GRP78 protein levels were analyzed in both liver types. For GRP78 mRNA expression in the liver, PCR fluorescent signals for GRP78 were standardized to PCR fluorescent signals obtained from an endogenous reference (β-actin). Comparative and relative quantifications of GRP78 gene products normalized to β-actin and control sham group were calculated by the 2^−ΔΔCT method. (b) p50ATF6α and p50ATF6β protein levels were analyzed in both liver types. For GRP78 and ATF6 protein levels in the liver, representative western blots at the top and densitometric analysis at the bottom. ^*P<0.05 versus sham; ⁺P<0.05 versus PH+I/R. ^#P<0.05 versus PH+I/R non-steatotic group. GRP78 mRNA and protein levels are significantly lower in the untreated steatotic livers group than in the untreated non-steatotic liver group (^#P<0.05)