Skip to main content
. Author manuscript; available in PMC: 2011 Feb 2.
Published in final edited form as: Biometals. 2009 Jan 8;22(1):109–115. doi: 10.1007/s10534-008-9194-z

Table 2.

V. cholerae ttpC complements ΔttpC mutation in V. anguillarum for growth around 2,3-DHBA

Strainsc Iron sourcesb
Ferric ammonium citrate Enterobactin 2,3-DHBA Vanchrobacin Hemin
V. anguillarum
775/pMMB208 +a + + + +
775 ΔttpC, ΔangA + − − − +
775 ΔttpC, ΔangA/pttpCva + + + + +
775 ΔttpC, ΔangA/pttpCvc + − + − +
775 ΔttpC, ΔangA/pttpCvv + − − − +
a

+ or − indicates growth or lack of growth around the specified iron source

b

1 µl of each iron source was spotted on the surface of the plates in the following concentrations; ferric ammonium citrate: 10 mg/ml; enterobactin: 1.0 mg/ml; 2,3-DHBA: 3.0 mg/ml; vanchrobactin: 1.0 mg/ml; hemin: 20 µg/ml

c

Mutations in chromosomally encoded genes were complemented by genes expressed from the low copy-number expression vector pMMB208. Expression was induced with 1 mM IPTG