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. Author manuscript; available in PMC: 2011 Feb 2.
Published in final edited form as: Biometals. 2009 Jan 8;22(1):109–115. doi: 10.1007/s10534-008-9194-z

Table 3.

TtpC is necessary for usage of iron sources when transport is mediated by the TonB2 system

Strainsc Iron sourcesb
TonB1- and TonB2-mediated transport TonB2-mediated transport
Ferric ammonium citrate Ferrichrome Enterobactin 2,3-DHBA
V. anguillarum
775/pMMB208 +a + + +
775 ΔtonB1, ΔttpC, ΔangA + − − −
775 ΔtonB1, ΔttpC, ΔangA/pttpCva + + + +
775 ΔtonB1, ΔttpC, ΔangA/pttpCvc + + − +
775 ΔtonB1, ΔttpC, ΔangA/pttpCvv + − − −
a

+ or − indicates growth or lack of growth around the specified iron source

b

1 µl of each iron source was spotted on the surface of the plates in the following concentrations; ferric ammonium citrate: 10 mg/ml; enterobactin: 1.0 mg/ml; 2,3-DHBA: 3.0 mg/ml; ferrichrome: 1.0 mg/ml

c

Mutations in chromosomally encoded genes were complemented by genes expressed from the low copy-number expression vector pMMB208. Expression was induced with 1 mM IPTG