Table 4.
In V. anguillarum, the TonB protein from E. coli does not require TtpC to mediate transport of the endogenous siderophore anguibactin
| Strainsc | Iron sourcesb | ||
|---|---|---|---|
| Ferric ammonium citrate | Enterobactin | Anguibactin | |
| V. anguillarum | |||
| 775/pMMB208 | +a | + | + |
| CSL68: ΔtonB1, ΔtonB2/pMMB208 | + | − | − |
| CSL64: ΔtonB1, ΔtonB2/pton8E. coli | + | − | + |
| 775 ΔttpC/pMMB208 | + | − | − |
| 775 ΔtonB1, ΔtonB2, ΔttpC/pMMB208 | + | − | − |
| 775 ΔtonB1, ΔtonB2, ΔttpC/ptonBE. coli | + | − | + |
a
+ or − indicates growth or lack of growth around the specified iron source
b
1 µl of each iron source was spotted on the surface of the plates in the following concentrations; ferric ammonium citrate: 10 mg/ml; enterobactin: 1.0 mg/ml; anguibactin: 1.0 mg/ml
c
Mutations in chromosomally encoded genes were complemented by genes expressed from the low copy-number expression vector pMMB208. Expression was induced with 1 mM IPTG