Table 1.
Bacterial strains used in the present study
| Organism and strain | Relevant characteristics | Source |
|---|---|---|
| E. coli | ||
| TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZ ΔM15 ΔlacX74 deoR recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
| S17-λpir | thi pro hsdR hsdM+ recA RP4-2-Tc::Mu-Km::Tn7 λ-pir | Laboratory stock |
| MM294 | F− endA1 hsdR17 supE44thi-1 λ− harbouring plasmid pRK2013 | Laboratory stock |
| BL21(DE3) | F−ompT[lon] hsdSB (rB − mB−) with DE3, λ prophage carrying the T7 RNA polymerase gene | Invitrogen. |
| DH5α | F−, φ80lacZ ΔM15, endA1, recA1, hsdR17, (rk−mk+), supE44, thi-1, gyrA96, relA1, Δ(lacZYA-argF)U169, λ− | Laboratory stock |
| KP1032 | W3110 tonB::kan | [45] |
| CSL-68 | KP1032/pMMB208 tonBEc | The present study |
| CSL-69 | KP1032/pMMB208 tonB2 V. anguillarum | The present study |
| CSL-70 | KP1032/pMMB208 | The present study |
| CSL-71 | KP1032/pMMB208 exbB2-exbD2-tonB2 | The present study |
| CSL-72 | KP1032/pMMB208 ttpC-exbB2-exbD2-tonB2 | The present study |
| CSL-73 | KP1032/pMMB208 tonB2Va-β4Ec | The present study |
| CSL-74 | KP1032/pMMB208 tonBEcΔβ4 | The present study |
| V. anguillarum | ||
| 775 (pJM1) | Wild-type Pacific Ocean prototype, O1 serotype | Laboratory stock |
| CSL-48 | 775 ΔtonB2 | [24] |
| CSL-52 | 775 ΔtonB2 ΔtonB1 | [24] |
| CSL-54 | CSL-48/pMMB208 wild type tonB2 | The present study |
| CSL-55 | CSL-48/pMMB208 Δ2-C tonB2 | The present study |
| CSL-56 | CSL-48/pMMB208 Δ3-C tonB2 | The present study |
| CSL-57 | CSL-48/pMMB208 Δ4-C tonB2 | The present study |
| CSL-60 | CSL-48/pMMB208 -AAA-C’ tonB2 | The present study |
| CSL-61 | CSL-48/pMMB208 -AAAAAA-C’ tonB2 | The present study |
| CSL-64 | CSL-52/pMMB208 tonBEc | The present study |
| CSL-65 | CSL-52/pMMB208 tonB2Va-β4Ec | The present study |
| CSL-67 | CSL-48/pMMB208 Δ179-195 loop3 | The present study |
| CSL-68 | CSL-48/pMMB208 Δ179-184 loop3 | The present study |
| CSL-69 | CSL-48/pMMB208 Δ185-190 loop3 | The present study |
| CSL-70 | CSL-48/pMMB208 Δ191-195 loop3 | The present study |