Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Plasmids used in the present study
| Plasmid | Relevant characteristic | Source |
|---|---|---|
| pDM4 | Suicide plasmid R6K origin CmR | [23] |
| pMMB208 |
IncQ, lacIq, Ωcat, ΩPtac, polylinker from M13mp19, CmR |
Laboratory stock |
| pCR2.1® | AmpR KmR | Invitrogen |
| pCR®-BluntII | KmR | Invitrogen |
| pET200 | Expression vector, KmR | Invitrogen |
| pCL22 | pET200 harbouring a partial tonB2 sequence (residues 76–206) |
The present study |
| pCL21 | pET200 harbouring a partial tonB2 sequence (residues 106–206) |
The present study |
