Fig. 5.

β2M antibody did not affect cell proliferation and endogenous AR expression; it also did not induce apoptotic death in human normal/nontumorigenic prostatic epithelial cells. A, β2M antibody (0-20 μg/mL, 3-d incubation) did not significantly affect cell proliferation of human normal prostatic epithelial cells, RWPE-1, as determined by mitochondrial MTS assay. Control IgG (20 μg/mL) also did not affect the growth of RWPE-1cells. The relative fold was assigned as 1.0 in the absence of β2M antibody treatment. Columns, mean of five replicate experiments; bars, SD. B, β2M antibody (0-10 μg/mL, 24-h treatment) did not inhibit AR nor activate cleaved caspase-9, caspase-3, and PARP protein expression in RWPE-1cells assayed by Western blot. Control IgG (10 μg/mL) also did not affect AR, cleaved caspase, or PARP protein expression.