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. Author manuscript; available in PMC: 2011 Feb 2.
Published in final edited form as: Science. 2010 May 20;328(5986):1689–1693. doi: 10.1126/science.1189731

Fig. 1.

Fig. 1

Increased expansion and cycling activity of nontransplanted Abca1−/− Abcg1−/− HSCs. (A) Quantification of LSK, CMP, GMP, and CLP compartments expressed as percentage of total BM or absolute numbers. DKO, double knockout. (B) Using flow cytometric analysis, LSK populations were subdivided into four populations based on differential expression of CD34 and CD150, and CD135 (Flt3) represents gated CD34+ CD150− LSK cells. (C) Quantification of LSK subpopulations from the most quiescent (long-term HSCs) to the most cycling LSK subset (short-term HSCs and multipotential progenitors) (CD34− CD150+ CD135− > CD34+ CD150+ CD135− > CD34+ CD150− CD135− > CD34+ CD150− CD135+) expressed as percentage of LSK population or whole BM. (D) Percentage and absolute number of LSK cells in S/G2M phase as determined by Hoechst staining and flow cytometric analysis. Data are means ± SEM (error bars) and are representatives of at least one experiment performed with five animals per group. *P < 0.05.