Fig. 4.

HDL rescue the myeloproliferative disorder and accelerated atherosclerosis of Abca1^−/− Abcg1^−/− BM transplanted mice. (A) Representative spleen, (B) heart, (C) liver, Peyer’s patches, and hematoxylin and eosin (H&E) staining of paraffin-embedded sections from high cholesterol–fed Ldlr^+/− and Ldlr^+/− apoA-1 transgenic recipient mice transplanted with Abca1^−/− Abcg1^−/− BM. (D) Representative H&E staining of the proximal aortas showing atherosclerotic lesions and correlation between mean lesion areas and leukocyte counts. (E) Lesion areas (individual and mean) were determined by morphometric analysis of H&E-stained sections. BM transplanted mice were fed a high-cholesterol diet for different time periods to approximately match lesion areas for mice not expressing apoA-1 transgene. (F) Leukocyte counts from WT and Abca1^−/− Abcg1^−/− transplanted recipients. Results are means ± SEM (error bars) of six to nine mice per group. *P < 0.05 apoA-I transgene effect; §P < 0.05 BM transplant effect.