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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Dev Biol. 2010 Dec 7;350(1):127–138. doi: 10.1016/j.ydbio.2010.11.028

Figure 7.

Figure 7

Lack of myelin formation and oligodendrocyte maturation in the absence of O1+ immature oligodendrocytes. In slice cultures (a-f) and dissociated cell cultures (g-i) the maturation of oligodendrocytes is dependent on the presence of O1+ cells. Few MBP+ cell bodies were found at P2+3 DIV (A, d) and P2+7 DIV (A, e) following elimination of O1+ cells. MBP+ processes failed to develop in treated cultures even at P2+14 DIV (A,f). Untreated slice cultures are shown in A, a-c. In mixed cell cultures (g-i) treated with O1 plus complement (O1+C), MBP+ cells were reduced and showed no extended MBP+ processes even at P2+14 DIV (A, j-l). In untreated cell cultures, advanced MBP+ cells were seen in increased numbers and had complex and extended processes (A, g-i). Quantitative analysis is shown in B for dissociated cell cultures and in C for brain slice cultures. *p<0.05 versus control; **p<0.01 versus control. Scale bar = 25μm.