Figure 6.

Immunofluorescence detection of relaxin protein in porcine oocytes and embryos. A minimum of 50 oocytes and embryos derived from 3 independent replicates were analyzed for immunofluorescence (IF). Micrographs show the IF detection (FITC, green color) of relaxin, which is homogenously distributed in oocytes and embryos. Nuclei are stained in blue with the Hoechst dye. The FITC signals in tested groups appear above the background as seen in the negative control group (NC). GV = Immature oocytes, MI = metaphase 1 oocytes, MII = metaphase II oocytes. Scale bars = 50 μm.