Table 1.

Porcine primer pair characteristics

Gene names	GenBank Acc. (NCBI)	Primer sequences (5'-3')	AT (oC)	Amplicon sizes (bp)
RXFP1	CA994862.1	S: AGGCTGACGAGGACAACT	52.5	132
		AS: CAGAACCGACCAAGCATT
RXFP2	CA997681.1	S: CATCTGCTGGATTCCCGTAT	55	117
		AS: TTCAAGGCACTGTTCACC
RELAXIN	NM213872.1	S: TGTGGCTCCGTCTCCTGGGG	55	164
		AS: GTTGCCTTCAGCTCCTGTGGC
β-ACTIN	U07786	S: 5'-ACTGGCATTGTCATGGACTCTG-3'	60	397
		AS: 5'-AGTTGAAGGTGGTCTCGTGGAT-3'

AT = Annealing temperature