Table 1.
Primers used for PCR amplification.
| Primer | Sequence (5′→3′) a | Locations b and modifications |
|---|---|---|
| PGFP-F | ggtacc ATGGGTAAAGGAGAAGAAC | 1aa;KpnI |
| PGFP-R | gagctc TTATTTGTATAGTTCATC | full-length reverse primer with stop codon; SacI |
| PS6-1-F | CG ggatcc ATGTCTGCCC | 1aa; BamHI |
| PS6-4-F | CTAG ccatgg GA ATGTCTGCCCACCTGACCAATTTAG | 1aa; NcoI |
| PS6-5-R | CG ggatcc TTACTCAGAGCTTAGTTGCCAGAGG | full-length reverse primer with stop codon; BamHI |
| PS6-6-R | CCG ctcgag CTCAGAGCTTAGTTGCC | full-length reverse primer without stop codon; XhoI |
| PS6-8-R | CCG ctcgag ATCAGCTACTTCGTCAG | 449aa; XhoI |
| PS6-9-F | CG ggatcc AC ATGTCTGCCCACCTG | 1aa; BamHI |
| PS6-10-F | CCG ctcgag ccatgg AAGCTTCTGATGTCCAG | 274aa; XhoI, NcoI |
| PS6-11-F | CCG ctcgag ccatgg ACTTGATTAATCATGCC | 395aa; XhoI, NcoI |
| PS6-12-R | CG ggatcc ggtacc ATCTCCAAAGTTAGCATCTAC | 703aa; BamHI, KpnI |
| PS6-15-R | CG ggatcc ggtacc CGTTTCATTAGCAGATGTTTTG | 659aa; BamHI, KpnI |
| PS6-16-R | TCC cccggg GAACAGATCGGCATGATTAATC | 403aa; SmaI |
| PS6-17-F | TCC cccggg GTGAATGATTTAACTGACGAAG | 440aa; SmaI |
| PS6-18-R | CATG gggccc GTCTTTCTCTTTTAGTAAAGAACAG | 615aa; ApaI |
| PS6-19-F | CATG gggccc TCTGCTAATGAAACGAATGATG | 655aa; ApaI |
| PS6-20-R | CATG gggccc GGCAATCTGTTCTTTAGCTTGTC | 580aa; ApaI |
| PS6-21-F | CATG gggccc GAGAACGAAATGTTGAAGGAACAG | 620aa; ApaI |
| PS6-24-F | GC tctaga ccatgg ACGTACTCAACCTGTCCAA | 98aa; XbaI, NcoI |
| PS6-25-F | GC tctaga ccatgg AAGCTTCTGATGTCCAGTC | 274aa; XbaI, NcoI |
| PS6-26-R | CCG ctcgag ggtacc CTCAGAGCTTAGTTGCCAGAG | full-length reverse primer without stop codon; XhoI KpnI |
| PS9-5-F | CTAG ccatgg GA ATGGCAGACCAAGAGCG | 1aa; NcoI |
| PS9-6-R | CG ggatcc AACGTCCAATTTCAAGG | full-length reverse primer without stop codon; BamHI |
| PS9-9-F | CG ggatcc ATGGCAGACC AAGAGCG | 1aa; BamHI |
| PS9-10-R | CCG ctcgag AACGTCCAATTTCAAGG | full-length reverse primer without stop codon; XhoI |
| PS9-11-F | CCG gaattc TCTCATCTCCCTAACC | 76aa; EcoRI |
| PS9-12-R | CG ggatcc CAAATACATTAAAAAGCC | 207aa; BamHI |
| PS9-13-F | CCG gaattc GGTGAAAATCCAAACTC | 208aa; EcoRI |
| PS9-14-R | CG ggatcc GTGATTAACTTCTTTATTTG | 248aa; BamHI |
| PS9-15-F | CCG ctcgag CT ATGGCAGACCAAGAGCG | 1aa; XhoI |
| PS9-16-R | ggtacc ggatcc TCAAACGT CCAATTTCAAG | full-length reverse primer, KpnI, BamHI |
| PS9-17-F | gaattc gtcgac ATGGCAGACCAAGAGC | 1aa, EcoRI, SalI |
| PS9-18-F | gaattc gtcgac ATGTCGTTGTTGCCAAT | 167aa, EcoRI, SalI |
| PS9-19-F | gaattc gtcgac ATGTATATAAAAGGCTT | 198aa, EcoRI, SalI |
a Introduced restriction endonuclease sites are in lower case. Two extra nucleotides (italicized) were added to allow in-frame expression of fusion proteins of interest.
b Numbered according to P6 amino acid sequence. The F or R designation in the primer names denotes whether the primer is a forward (5′) or reverse (3′) primer, respectively.