Table 2.
Comparison of the hatchability of new recombinant viruses in embryonated chicken eggs vs. the viruses with wild type HAs and the optimization of the dose and timing for in-ovo vaccination.
| Vaccine | Dose (EID50) | Embryo age (Day) | % Hatchability (# hatched/total #) | |
|---|---|---|---|---|
| H7N2 (VIVA/04) Vaccine | 2mH7N2:6WF10 att | 106 | 18 | 50% (15/30) |
| (P = 0.016) | ||||
| 106 | 19 | 93% (42/45) | ||
| (P = 0.061) | ||||
| 107 | 19 | 80% (24/30) | ||
| (P = 0.066) | ||||
| 2H7N2:6WF10att | 106 | 18 | 30% (9/30) | |
| 106 | 19 | 43% (13/30) | ||
| 107 | 19 | 37% (11/30) | ||
| H9N2 (WF10) Vaccine | 2mH9N2:6WF10att | 106 | 18 | 63% (19/30) |
| (P = 0.0161) | ||||
| 106 | 19 | 90% (27/30) | ||
| (P = 0.260) | ||||
| 107 | 19 | 83% (25/30) | ||
| (P = 0.154) | ||||
| 2H9N2:6WF10att | 106 | 18 | 37% (11/30) | |
| 106 | 19 | 60% (18/30) | ||
| 107 | 19 | 37% (11/30) | ||
| PBS (Mock) | 0 | 18 | 93% (28/30) | |
| 0 | 19 | 96% (43/45) | ||
* 3 chickens dead at 2-5 days post-hatching.