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. 2011 Feb 2;6(2):e15820. doi: 10.1371/journal.pone.0015820

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Vitali et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) 180 minutes of continuous WFMP-FLIM exposure of a living HeLa cell expressing a tagRFP-5AA-eGFP binary construct by pulsed interleaved excitation does not induce any detectable photobleaching. The two image streams are pseudo colored: eGFP (green) and tagRFP spectral detection channels (red). (b) Fluorescence decays in the 473GFP (green), 473RFP (red) and (c) 532GFP (green) and 532RFP (red) detection channels. The 473GFP/473RFP and the 532GFP/532RFP channels have been merged in pseudo-color images.