Figure 6. HDAC inhibition reduces SRp40 association with the EDB exon.

(A) SRp40-GFP stably expressed from a BAC cell line was immunoprecipitated using an anti-GFP antibody. SRp40 interaction with the FN1 gene was measured in cells treated for 6 h with NaB and in control cells. No changes in distribution of another splicing regulator PTB (B) or general splicing factors Sm proteins (C) were detected. Probes detecting gene loci around alternative EDB exon are shaded. The average of 3-5 experiments is shown, including SEM, ** indicates p≤0.01 and * p≤0.05 of the t-test.