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. 2011 Feb 2;6(2):e16642. doi: 10.1371/journal.pone.0016642

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Sun et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Small brown spots around S180 tumor cells indicated antibody-reactive T cells. (A) CD4-positive cells detected by rabbit CD4-specific polyclonal antibody. (B) CD8 positive cells detected by rabbit CD8-specific polyclonal antibody. (C) SEA-reactive T cells. Sections were incubated with a SEA solution, followed by mouse polyclonal anti-SEA IgG fraction and anti-mouse secondary antibody. Microscope magnification: ×400 in A to C.